Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 10;9:5848. doi: 10.1038/s41598-019-42283-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Lrrc75a-as1 inhibits vascular calcification. (A) Measurement of calcium deposition after overexpression of candidate lncRNAs. Compared to other lncRNAs, overexpression of Lrrc75a-as1 most significantly changed the calcium content. Error bars indicate standard error between four independent experiments with triplicates in each experiment. P value was calculated by a two-sided paired t-test. (B) Expression change of the osteoblast-related factors after Lrrc75a-as1 overexpression. The expression of osteoblast-related factors, including Runx2, Msx2, and Bmp2 was measured. Error bars indicate standard error between three independent experiments. (C) Measurement of calcium deposition after knockdown of Lrrc75a-as1. Six independent experiments were performed, and the P value was calculated by a two-sided paired t-test. (D) Determination of calcification with Alizarin red S staining. After the knockdown of Lrrc75a-as1, the calcium deposits were measured with Alizarin red S dye. The pictures of cell culture dishes and microscopic images (50×) were shown. The amounts of Alizarin red S-stained mineralization were quantified from three cell culture dishes and error bars indicate the standard errors. (E) Working model. The expression level of Lrrc75a-as1 is reduced during vascular calcification in VSMCs. Upregulation of Lrrc75a-as1 expression may switch the VSMC phenotype from an osteoblastic/chondrogenic to a contractile phenotype, and mitigate calcium accumulation.