Table 3.
Cell-free plasma microRNAs with differential expression in the discovery cohort.
| miRNA | Outcome | TIME POINT A | TIME POINT B | TIME POINT C | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Mean relative expression | SEM | P value | Mean relative expression | SEM | P value | Mean relative expression | SEM | P value | ||
| hsa-miR-150-5p | Outcome 1- TERM | 1.0 | 0.3 | 1.0 | 0.2 | 1.0 | 0.1 | |||
| Outcome 2- PRETERM | 6.2 | 3.1 | 0.04 | 8.8 | 3.9 | 0.05 | 15.4 | 5.7 | 0.005 | |
| Outcome 3- SHORT | 4.5 | 1.7 | 0.05 | 6.2 | 2.0 | 0.02 | 9.3 | 2.6 | 0.005 | |
| hsa-miR-374a-5p | Outcome 1- TERM | 1.0 | 0.4 | 1.0 | 0.2 | 1.0 | 0.3 | |||
| Outcome 2- PRETERM | 6.2 | 3.7 | 0.04 | 8.2 | 4.3 | 0.03 | 11.4 | 7.0 | 0.05 | |
| Outcome 3- SHORT | 5.8 | 2.4 | 0.04 | 9.5 | 3.6 | 0.009 | 8.6 | 3.9 | 0.03 | |
| hsa-miR-19b-3p | Outcome 1- TERM | 1.0 | 0.3 | 1.0 | 0.2 | 1.0 | 0.2 | |||
| Outcome 2- PRETERM | 4.1 | 1.6 | 0.03 | 4.5 | 1.6 | 0.03 | 8.0 | 4.2 | 0.05 | |
| Outcome 3- SHORT | 6.1 | 3.1 | 0.01 | 5.7 | 1.5 | 0.004 | 6.2 | 2.3 | 0.08 | |
| hsa-miR-185-5p | Outcome 1- TERM | 1.0 | 0.2 | 1.0 | 0.3 | 1.0 | 0.2 | |||
| Outcome 2- PRETERM | 6.2 | 2.7 | 0.03 | 4.5 | 1.5 | 0.08 | 8.8 | 1.9 | 0.002 | |
| Outcome 3- SHORT | 4.9 | 1.5 | 0.05 | 6.8 | 2.9 | 0.04 | 5.6 | 1.3 | 0.01 | |
| hsa-miR-15b-5p | Outcome 1- TERM | 1.0 | 0.3 | 1.0 | 0.2 | 1.0 | 0.2 | |||
| Outcome 2- PRETERM | 11.3 | 8.8 | 0.03 | 8.1 | 4.2 | 0.04 | 17.4 | 6.1 | 0.04 | |
| Outcome 3- SHORT | 7.5 | 4.4 | ns | 8.0 | 3.0 | 0.02 | 11.4 | 3.1 | 0.02 | |
| hsa-miR-191-5p | Outcome 1- TERM | 1.0 | 0.4 | 1.0 | 0.3 | 1.0 | 0.1 | |||
| Outcome 2- PRETERM | 5.5 | 5.2 | ns | 7.0 | 4.0 | 0.04 | 6.6 | 5.3 | 0.04 | |
| Outcome 3- SHORT | 4.0 | 2.8 | ns | 9.2 | 4.5 | ns | 10.7 | 2.6 | 0.04 | |
| hsa-miR-93-5p | Outcome 1- TERM | 1.0 | 0.2 | 1.0 | 0.1 | 1.0 | 0.2 | |||
| Outcome 2- PRETERM | 4.5 | 2.6 | ns | 5.3 | 2.2 | ns | 6.6 | 2.6 | 0.02 | |
| Outcome 3- SHORT | 3.9 | 1.3 | ns | 5.1 | 1.2 | 0.004 | 5.1 | 1.6 | 0.05 | |
| hsa-let-7a-5p | Outcome 1- TERM | 1.0 | 0.2 | 1.0 | 0.1 | 1.0 | 0.1 | |||
| Outcome 2- PRETERM | 6.8 | 4.7 | ns | 10.5 | 4.9 | 0.06 | 24.2 | 8.1 | 0.004 | |
| Outcome 3- SHORT | 5.4 | 2.4 | ns | 8.6 | 2.7 | ns | 14.3 | 3.6 | 0.01 | |
| hsa-miR-23a-3p | Outcome 1- TERM | 1.0 | 0.2 | 1.0 | 0.2 | 1.0 | 0.1 | |||
| Outcome 2- PRETERM | 14.1 | 10.1 | ns | 6.5 | 2.2 | 0.004 | 21.2 | 9.3 | 0.02 | |
| Outcome 3- SHORT | 8.8 | 4.9 | ns | 8.0 | 3.4 | 0.02 | 12.8 | 4.1 | 0.04 | |
Expression of specific miRNAs was measured using RT-PCR in women who had normal cervical length and delivered at term (Outcome 1) and was compared to women who delivered preterm (Outcome 2) or women who developed early cervical shortening (Outcome 3). Results are presented as fold change, mean relative expression and IQR at each gestational time-point (time point A:12–14+6 weeks, time point B: 15–18+6 weeks and time point C :18–21+6 weeks).