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. 2019 Apr 4;13:120. doi: 10.3389/fncel.2019.00120

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Copyright © 2019 Vantaggiato, Castelli, Giovarelli, Orso, Bassi, Clementi and De Palma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alterations in Drp1 levels and function affect final neuronal differentiation. (A) Drp1wt, Drp1K38A, Drp1 shRNA and control clones were induced to differentiate with RA. RNA was extracted every day from d5 to d8 and used to analyze Tubulin β-III, MAP2 and Gap43 expression levels by Real-Time PCR. Undifferentiated P19 cells stably transfected with the vector alone were used as endogenous control. Results are expressed as fold increase of endogenous control and reported as individual data plus the mean ± SEM (two-way ANOVA followed by Tukey’s multiple comparison test, n = 6). * vs ctr (***p < 0.001); ⁺ vs Drp1wt (⁺⁺⁺p < 0.001). Lines indicate samples to whom symbols are referred. (B) Drp1wt, Drp1K38A, Drp1 shRNA and control clones were induced to differentiate with RA and on d6 neurons were fixed and stained with anti-Tubulin β-III, MAP2 and Gap43 Abs (green). Scale bar: 50 μm.