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. 2019 Apr 4;10:664. doi: 10.3389/fimmu.2019.00664

Figure 3.

Figure 3

Overexpression of SLPI reduces the BzATP-mediated IL-1β release by U937 cells. Human monocytic U937 cells were left untreated, transfected with the empty vector (EV), or with an expression vector containing the coding sequence for human SLPI (SLPI). Twenty-four hours after transfection, the cells were primed with LPS (1 μg/ml, for 5 h) and further stimulated with 2′(3′)-O-(4-benzoylbenzoyl)adenosine 5′-triphosphate triethylammonium salt (BzATP; 100 mM) for 30 min. The concentration of IL-1β in cell culture supernatants was measured by ELISA. The mRNA expression of SLPI or pro-IL-1β was assessed by real-time PCR. (A) Transfection with SLPI expression vector increased the mRNA level of SLPI compared to cells transfected with EV. (B) The BzATP-induced IL-1β release was significantly lower in cells overexpressing SLPI compared to cells transfected with EV. (C) The mRNA level of pro-IL-1β was not affected by SLPI overexpression; *p ≤ 0.05 significantly different compared to cells transfected with EV (A) or transfected with EV and stimulated with LPS and BzATP (B,C). Experimental groups were compared by Kruskal-Wallis test followed by Mann-Whitney rank sum test.