Skip to main content
. 2019 Apr 4;10:378. doi: 10.3389/fpls.2019.00378

FIGURE 7.

FIGURE 7

13C and15N uptake rates as ATP/CTP equivalents of excised poplar roots achieved from 13C/15N labeled ATP or CTP. Excised poplar roots were taken from poplar plants grown with 0.25 mM Pi (July). 13C and 15N uptake rates calculated as ATP and CTP equivalents were analyzed by the application of 0.169 mM labeled ATP-13C1015N5 (10 atom%) or CTP-13C915N3 (10 atom%). The competition with Pi was tested by the addition of 0.25 mM Pi. The effect of acid phosphatases was investigated by the simultaneous application of nucleotides plus 0.5 mM MoO42-. Data presented are box plots for 13C uptake rates as ATP equivalents (A) and 13C uptake rates as CTP equivalents (B). Left to the box plots individual values achieved from single incubation cambers are presented (n = 4). 15N uptake rates as ATP and CTP equivalents were similar to the 13C uptake rates as ATP and CTP equivalents. Significant differences were analyzed by One Way ANOVA followed by the Post hoc tests Bonferroni and Tukey with p < 0.05 but, statistically significant differences were not found. Correlations over all treatments between 13C and 15N uptake rates are provided for ATP (C) and CTP (D). Slopes and Pearson correlation coefficients are given in Table 1.