Figure 4.

EGT cancels TAM proliferation induced by TLR2/6 ligand. (A,B) LLC-OVA-implanted WT B6 mice were treated with PBS, EGT, and/or Pam2CSK4 + OVA, as per Figure 1A and tumors were harvested at day 18. In (B), mice were injected with Pam2CSK4 without OVA. Tumor-infiltrating cells were analyzed by flow cytometry to count the number of CD11b⁺ Gr-1^−/low F4/80⁺ cells. Representative data are shown as FACS plot. n = 4–5 mice per group. (C) Sorted tumor-infiltrating F4/80⁺ cells were cultured with or without EGT for 24 h, and then, cells were stimulated with 50 nM of Pam2CSK4. WST-1 assay was performed 72 h after Pam2CSK4 treatment. n = 3. *P < 0.05. n.s., not significant.