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. 2019 Apr 10;18:100634. doi: 10.1016/j.bbrep.2019.100634

Fig. 4.

Fig. 4

Linker histone H1 binding to the placeholder nucleosome. A. Schematic representation of the H1 binding assay. In this assay, increasing amounts of H1 are mixed with the nucleosomes in the presence of Nap1. After an incubation, the complex formation is analyzed by native-PAGE. B. The predicted nucleosome positioning on the 193 base-pair DNA is presented by an ellipse. A gray box indicates the 145 base-pair Widom 601 sequence region. C. A representative gel image of the H1 binding assay. Increasing amounts of H1 (0, 0.23, 0.32, 0.41, 0.50, and 0.60 μM) were mixed with the canonical (lanes 1–6), H2A.Z (lanes 7–12), and placeholder (lanes 13–18) nucleosomes (0.1 μM) containing the 193 base-pair Widom 601 DNA in the presence of Nap1 (0.3 μM). After an incubation at 25 °C for 30 min, the reaction mixtures were analyzed by native-PAGE with ethidium bromide staining.