Figure 2.

A: Representative fundus images with fluorescein angiography (FA) at the indicated time points. Both FH^W/W and FH^R/R/C6^−/− mice show normal fundus and FA images. An FH^R/R mouse had almost no perfusion up until 90 seconds, whereas an FH^R/R/C5aR1^−/− mouse had delayed and incomplete retinal perfusion and fluorescence leakage around the optic nerve head. B: Quantification of abnormalities on fundus images and FA images. Abnormalities, including vessel occlusion, white spots, ischemic patches on fundus images, and perfusion delay and vascular leakage on FA images, are observed in FH^R/R and FH^R/R/C5aR1^−/− mice, but not in FH^W/W and FH^R/R/C6^−/− mice. Only one anti-C5 monoclonal antibody (mAb)–treated FH^R/R mouse shows vessel occlusion and white spots; the others exhibit no pathologic changes. Fractions represent ratios of abnormal mice/all experimental mice for each genotype. Fisher exact test reveals a significant association between genotype and likelihood of exhibiting all of the observed pathologic changes. Dotted lines separate different graphs. Bars within each graph are displayed in the same order as genotypes in the key. n = 17 FH^R/R mice (B); n = 14 FH^R/R/C5aR1^−/− mice (B); n = 8 FH^W/W mice (B); n = 15 FH^R/R/C6^−/− mice (B); n = 5 anti-C5 mAb–treated FH^R/R mouse (B). ^∗∗∗P < 0.001, ^∗∗∗∗P < 0.0001.