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. 2018 Dec 21;25(5):875–885. doi: 10.1093/ibd/izy386

FIGURE 6.

FIGURE 6.

Comparison between E. coli NC101 and K12 for in vitro activation of CD4+ MLN cells. CD4+ MLN cells from either E. coli NC101 (A and B)– or K12 (C and D)–monoassociated IL-10-/- mice were co-cultured with bacterial lysate–pulsed APCs for 72 hours. The E. coli lysate used to pulse APCs is identified below the x-axis. The amounts of IFN-γ (A and C) and IL-17 (B and D) secreted into culture supernatants were measured by ELISA. Each point represents the amount of IFN-γ or IL-17 in the mean of the triplicate supernatants of CD4+ MLN cells from an individual mouse. Points connected by lines indicate results for an individual mouse.