Fig. 6.

The combination of PGC-1β and FOXA2 inhibits breast tumor growth and metastasis through regulating the PI3K-AKT-mTOR signaling pathway. a The related proteins of PI3K-AKT-mTOR signal pathway were examined by western blot. GAPDH was used as loading control. b The related proteins of PI3K-AKT-mTOR signal pathway were evaluated in MCF-7 cells when co-transfected with sh-PGC-1β and FOXA2. c The protein levels of PI3K, p-PI3K, AKT, p-AKT, mTOR were detected after treatment of mTOR inhibitor rapamycin (1000 nM). Data are shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001