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. 2019 Apr 11;10:8. doi: 10.1186/s13227-019-0119-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Lipid and fatty acid composition of A. mollis. a Neutral lipid content of triacylglycerol (TAG), free fatty acid (FFA) and diacylglycerol (DAG) in auricularia, auricularia with hyaline spheres (HS) and through metamorphosis via doliolaria to pentactula. (N = 5). b Total structural lipids (phospholipids and sterol) as in Panel A, but with age of larvae (days post-fertilization) as X-axis. c TLC plate developed in a neutral solvent system containing hexane–diethyl ether–acetic acid (80:20:1, by vol.) followed by staining with iodine vapours. Lipid extracts of A. mollis auricularia with HS (A-HS), doliolaria (D) and lipid standards (STD). Grey line shows origin. d Re-extracted TAG band from TLC plate stained with Nile Red shows yellow colour under blue light excitation. e Re-extracted FFA band from TLC plate stained with Nile Red shows red colour under blue light excitation. f Fatty acid composition of auricularia and auricularia with hyaline spheres (HS) divided into saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids (N = 3)