Table 1.

Primers for species identification used for PET-PCR

Primer name	Sequence (5′–3′)
Original genus 18sFor	GGC CTA ACA TGG CTA TGA CG
Original genus FAM 18sRev	FAM-aggcgcatagcgcctggCTGCCTTCCT TAG ATGTGG TAG CT
Falciparum For	ACC CCTCGCCTG GTG TTT TT
Falciparum Rev	HEX-aggcggataccgcctggTCGG GCC CCA AAA ATA GGA A
P. vivax For	GTA GCC TAAGAAGGC CGT GT
P. vivax Rev	HEX-aggcgcatagcgcctggCCTGGGG GAT GAA TAT CTC TAC AGC ACT GT
P. malariae For	AAGGCAGTAACACCAGCAGTA
P. malariae Rev (based on dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene)	FAM-aggcgcatagcgcctggTCCCATGAAGTTATATTCCCGCTC
P. ovale For	FAM-aggcgcatagcgcctggCCACAGATAAGAAGTCTCAAGTACGATATT
P. ovale Rev	TTGGAGCACTTTTGTTTGCAA

Table showing forward and reverse primers for species identification used in PET-PCT assay