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. 2019 Apr 11;38:155. doi: 10.1186/s13046-019-1152-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — SNH beneficially reduces EMT progression in NSCLC cells. a. Chemical structure of SNH. b. CCK-8 proliferation assay of A549, NCI-H460 and NCI-H1299 cells treated with different concentrations of SNH for 24 H, 48 H or 72 H. c. Wound scratch assay of A549 and NCI-H1299 cells treated with SNH for 24 H. d. Transwell invasion assays showed that SNH decreased the invasion ability of A549 and NCI-H1299 cells. e. mRNA level of EMT markers in SNH-treated A549 and NCI-H1299 cells as determined by qRT-PCR. f. Expression of EMT markers in SNH-treated A549 and NCI-H1299 cells as determined by western blot analysis. g. Immunofluorescence staining of E-cadherin expression in SNH-treated A549 and NCI-H1299 cells. The bars and error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001