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NOX1 and NOX4 are involved in DEX-induced ROS production. (a–c) PCR confirmation of NOX1 (a), NOX4 (b), and NOX2 (c) silencing in MC3T3-E1 cells transiently transfected with siRNA (60 nM) for 48 h. (d) DCFH-DA probe detection of intracellular ROS in siRNA-transfected osteoblasts treated with 1000 nM DEX for 24 h. (e) Quantitative analysis of DCF fluorescence intensity in (d) (n = 3; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001).
