Figure 1.

Application of RIC to HEK293 Cells Infected with SINV

(A) Schematic representation of cRIC.

(B) Schematic representation of SINV and chimeric SINV-mCherry genomes.

(C) Analysis of the proteins synthesized in uninfected and SINV-infected HEK293 cells by [³⁵S]-Met/Cys incorporation for 1 h followed by autoradiography.

(D) Analysis of total and phosphorylated eIF2α by western blotting.

(E) Silver staining analysis of the “inputs” (i.e., total proteome, left) and eluates (i.e., RBPome, right) of a representative RIC experiment in SINV-infected cells.

(F) qRT-PCR analysis of the eluates of a representative RIC experiment using specific primers against SINV RNAs, actb and gapdh (for normalization) mRNAs. Error bars represent SE.

hpi, hours post-infection; MW, molecular weight.

See also Figure S1.