Figure 2.
Analysis of the RNA-Bound Proteome in SINV-Infected HEK293 Cells by cRIC
(A) Scatterplot showing the intensity ratio between 4 hpi and uninfected conditions of each protein (dots) in the eluates of two biological replicates of cRIC.
(B) Volcano plot showing the log2 fold change and the significance (p value) of each protein between 4 hpi and uninfected conditions using data from three biological replicates.
(C) As in (A) but for 18 hpi.
(D) As in (B) but for 18 hpi.
(E) Western blotting analysis with specific antibodies of the eluates of a representative RIC experiment in SINV-infected HEK293 cells.
(F) Molecular function (top) and cellular component (bottom) Gene Ontology (GO) term enrichment analysis of the stimulated (salmon) against inhibited (blue) RBPs (18 hpi).
(G) Representative scatterplot comparing the raw intensity of each protein in the eluates of two cRIC replicates at 18 hpi.
FDR, false discovery rate; n.s., non-significant.