Fig. 1.

Sostdc1 protein levels are increased by myeloma cells-OB progenitor interaction and blocking Sostdc1 reverses myeloma-induced suppression of OB differentiation: (A) Flow cytometry analysis of 5TGM1-GFP cells and OB progenitors (alone or in co-culture) for the expression of murine Sostdc1 protein. (B) Western blot analysis of cell lysates from 5TGM1-GFP cells and OB progenitors (alone or in co-culture) illustrating the level of Sostdc1 protein. The relative intensity (RI) of the Sostdc1 protein band was normalised to the RI of corresponding GAPDH protein band in the same sample. HK2 cells were used as a positive control for Sostdc1 expression. (C) Quantitative RT-PCR analysis of Runx2 gene expression in 5TGM1-GFP cells and OB progenitors (alone or in co-culture), in the absence or presence of anti-Sostdc1 antibody. N = 3 independent experiments, Student t-test, One-way ANOVA, *P < 0.05.