Fig. 2.

Sostdc1 was upregulated in 5TGM1 tibiae sections and 5TGM1-OB progenitor co-cultures: Immunoassays and end-point PCR were performed to detect Sostdc1 in 5TGM1-GFP cells and differentiating OB progenitors (alone and in co-culture). (A) Immunohistochemistry for Sostdc1 in naïve and 5TGM1-infiltrated tibia sections. Arrows indicate osteoblasts (naïve, left image) or tumour cells (5TGM1, right image). (B) Confocal microscopy for Sostdc1 in OB progenitors cultured alone (i) 5TGM1-GFP (CD138 positive cells) cultured alone (ii) or in co-culture (iii). 5TGM1/OB progenitors in contact highlighted in white squares. (C) High power field of co-cultures stained with DAPI and with antibodies for CD138/Sostdc1 merged. Isolated and associated cells are highlighted. All figures are representative of 3 independent experiments.