Fig. 3.

Sostdc1 reduces OB progenitor differentiation induced by Wnt and BMP ligands: Differentiating OB progenitors were treated with Wnt3a, BMP2 or BMP7. Dkk1 and noggin were used as known Wnt and BMP antagonist controls, respectively. Quantitative RT-PCR of Runx2 mRNA relative expression to B2M in differentiating OB progenitors treated with Wnt3a (A), BMP2 (B) and BMP7 (C) alone or in the absence or presence of Sostdc1, Dkk1 or noggin. Mineralisation of OB progenitors treated with Wnt3a (D), BMP2 (E) and BMP7 (F) in the absence or presence of Sostdc1, Dkk1 or Noggin. Results are expressed as the % area of the well that was mineralised. N = 4 independent experiments. One-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001.