PCF11 Enhances Transcription Termination Genome-wide
(A) Genomic profile of TSC22D1. Gray shading: termination window in control cells (siLUC, black). PCF11 depletion (siPCF11) is depicted in red. For chrRNA-seq and mNET-seq only sense strand is shown; ChIP is not strand specific. In all profiles, numbers in brackets indicate the viewing range (rpm).
(B–D) Metagene analysis of total Pol II ChIP-seq (B), T4ph mNET-seq (C), and CPSF73 ChIP-seq (D) in cells ± siPCF11 on pc genes >5 kb long with PAS separated by >6 kb from the nearest gene on the same strand (n = 8,389).