Figure 3.
Genomic Pattern of PCF11 Binding
(A) Epitopes recognized by PCF11 antibodies; α-PCF11-Int binds an Internal peptide, and α-PCF11-Ct the C terminus. Amino acid (AA) numbering corresponds to the main human PCF11 isoform NP_056969.2.
(B) Genomic profile of PCF11 binding to ACTB. PCF11-(Int+Ct) corresponds to merged antibody profiles. Blue bars below PCF11 ChIP-seq signal indicate PCF11 enrichment.
(C) Enrichment definition: regions with ChIP-seq signal significantly above background for both PCF11 antibodies were considered PCF11-enriched.
(D) Heatmap of PCF11-(Int+Ct) ChIP-seq signal (log2IP/input) across pc genes ranked from highest to lowest PCF11 signal.
(E and F) Meta-gene analysis of PCF11 binding on pc genes (n = 8,389). Plotted is PCF11 ChIP-seq signal relative to input (E) and Pol II (F). PCF11 and Pol II signals were calculated as log2(IP/input).
(G and H) Genomic profiles showing PCF11 binding and activities on MALAT1 (G) and MIR17HG (H).