Figure 2.

Workflow of the device. In step 1, whole blood is collected into the lysis buffer by the capillary tube. In step 2, the lysate is loaded into the binding chamber of the four testing units. In step 3, the disc is sealed and inserted into the analyzer, which then performs automated sample preparation and amplification. This automated process consists of four steps: binding (3 min), washing (4 min), elution (3 min) and amplification (40 min). During the amplification process, the real-time fluorescence signal from each testing unit was recorded and analyzed. Finally, in step 4, the testing results are reported.