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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: J Hepatol. 2018 Oct 1;70(1):108–117. doi: 10.1016/j.jhep.2018.09.023

Fig. 5. Loss of β-catenin protects mice from DDC-mediated depletion of heme.

Fig. 5.

(A) In-gel heme staining shows decreased protein-bound heme in control +DDC but not in β-catenin DsiRNA+DDC. Top panels: arrowheads represent bottom of well, stacking-resolving gel junction, and end of gel, respectively. Bottom panels: arrowheads represent stacking-resolving gel junction. (B) Quantification of in-gel heme staining demonstrates loss of heme-bound proteins in control +DDC diet compared to β-catenin DsiRNA+DDC and untreated controls; **p <0.01 vs. control DsiRNA (t test). (C) Measurement of total heme content shows persistence of heme in β-catenin DsiRNA+DDC compared to control+DDC; %p <0.05 vs. control+DDC (t test). (D) HO-1 mRNA expression is suppressed in the absence of β-catenin before and after DDC compared to controls; *p <0.05 vs. control DsiRNA, #p <0.05 vs. β-catenin DsiRNA, %p <0.05 vs. control+DDC (t test). For (E) and (F), n = 3 mice per group. DDC, 3,5-diethoxycarbonyl-1,4-dihydrocollidine; DsiRNA, dicer-substrate RNA.