Figure 3. Mineralization potential of APC assessed through calcium deposition by using Alizarin Red S staining. Once kept in contact with CH or mTAP at 250 µg/mL for 5 days, osteogenic mineralization was induced for 14 and 21 days. A – macroscopic view of wells after staining; and B – quantification of calcium eluates normalized by positive control (DM). PM: proliferation medium, DM: differentiation medium; CH + DM and mTAP + DM: calcium hydroxide or mTAP prior to mineralization; Med + DM: medication prior to mineralization. **P<0.01 and ***P<0.001 in comparison to PM; #P<0.05 and ###P<0.001 in comparison to DM (n=9).
