FIG. 2.

Binding of p53 and acetylated H3K9 at p66Shc promoter. HRECs, incubated in high glucose for 96 h, were analyzed for (a) p53 binding and (b) H3K9Ac by ChIP technique using IgG as an antibody control (indicated as ^). Each measurement was made in duplicate in three to four samples per group, and values obtained from cells in 5 mM glucose are considered as 1.5 and 20 mM representing 5 or 20 mM glucose; l-Glu represents 20 mM l-glucose; Sirt and R represent cells transfected with Sirt1 cDNA or with transfection reagent alone, and incubated in 20 mM glucose for 96 h. * and ^#p < 0.05 compared with cells in 5 or 20 mM glucose, respectively. ChIP, chromatin immunoprecipitation; H3K9Ac, histone 3 lysine 9 acetylation; Sirt1, sirtuin 1.