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. 2019 Mar 19;30(13):1621–1634. doi: 10.1089/ars.2018.7542

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Copyright 2019, Mary Ann Liebert, Inc., publishers

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FIG. 8. — Mitochondrial localization of p66Shc in diabetes. (a) Mitochondrial localization of p66Shc was quantified in the rat retinal cryosections by immunofluorescence staining using Texas Red and DyLight 488 labeled secondary antibodies for p66Shc and CoxIV (mitochondrial marker), respectively. (b) Pin1p-p66Shc colocalization was quantified by immunofluorescence staining using Texas Red and DyLight 488 labeled secondary antibodies, respectively, the insets show magnified vascular areas. (c) Pin1 expression in retinal microvessels (hypotonic shock) was quantified by qPCR using β-Actin as a housekeeping gene. Values are represented as mean ± SD from five to six rats per group. *p < 0.05 compared with normal.