Fig. 4.

Lipopolysaccharide (LPS)-induced priming of bone marrow-derived macrophages (BMDMs) was decreased in ASK-1-deficient cells. BMDMs were isolated and treated with LPS for 2, 4, or 6 h. A: representative Western blots of NOD-like receptor 3 (NLRP3), pro-IL-1β, and procaspase-1 after LPS treatment of BMDMs. GAPDH was used as a loading control, and densitometry values were normalized to GAPDH. Each protein indicated was immunoblotted separately from independent gels, and each set of bands for a given protein was cropped from the same blot. Densitometry analysis is shown for NLRP3 (B), pro-IL-1β (C), and procaspase-1 (D) expression following LPS stimulation in wild-type and apoptosis signal-regulating kinase-1-deficient (ASK1^−/−) BMDMs. P < 0.05, significant difference from wild type (*) and untreated (#) (n = 8–9 for B, 5–7 for C, and 6–7 for D).