The effects of miR-451a on mitochondria bioenergetic profile in diabetic conditions. A: the injection of reagents (oligomycin, FCCP, RAA) during the Seahorse analysis was represented with the indicated lines, and their effects on the average OCR was measured in the bioenergetic profile in normal culture (nondiabetic) conditions. Basal levels of OCR (B) and ATP production (C) were calculated and compared. D: representative trace identifying the OCR after 4-HNE treated (10 μM, 24 h). Basal levels of OCR (E) and ATP production (F) were calculated and compared in 4-HNE conditions (mean ± SE, n = 4–7, *P < 0.05).