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. 2018 Dec 21;316(3):E373–E382. doi: 10.1152/ajpendo.00244.2018

Fig. 3.

Fig. 3.

Effect of miR-370-3p on steroidogenic factor 1 (SF-1) expression. A: overexpression of miR-370-3p suppressed SF-1 protein expression. B: knockdown of miR-370-3p enhanced SF-1 protein expression level in endometriotic cyst stromal cells (ECSCs). Quantification of protein bands measured by densitometry and normalized to respective GAPDH protein bands is shown in bar graphs. Bar graphs represent the average values of 3 individual experiments (blots). *P < 0.05, statistical significance compared with inhibitor negative control (NC). **P < 0.01, statistical significance compared with mimic NC. C and D: effect of miR-370-3p on SF-1 downstream genes steroidogenic acute regulatory protein (StAR) and CYP19A1 expression and cell proliferation in ECSCs. C: quantitative RT-PCR data showing significant decrease in the mRNA levels of StAR and CYP19A1 in cells transfected with miR-370-3p mimic compared with either treatment with the scrambled control or untreated controls. D: StAR and CYP19A1 mRNA levels were significantly upregulated by overexpression of miR-370-3p inhibitor compared with the controls. Each bar represents the means ± SE for data from 3 individual experiments, and each experiment was performed in duplicate. *P < 0.05, statistical significance compared with control. **P < 0.01, statistical significance compared with control.