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. 2019 Mar 12;10(21):2068–2085. doi: 10.18632/oncotarget.26699

Figure 4. OSM induces IL-6 secretion in an ER-dependent manner.

Figure 4

(A) IL-6 secretion levels were measured by ELISA on CM collected from various OSM-treated cells. ER− MDA-MB-231, MDA-MB-468, and 4T1.2 cells display high (over 4-fold) levels of OSM-induced IL-6 secretion, while ER+ MCF7, and T47D cells do not. (B) OSM also induces IL-6 secretion in PC3 and DU145 (prostate) and HeLa (ovarian) ER− non-breast cancer cell types. (C) MDA-MB-231 cells were transfected with an ERα expression vector, and the presence of ERα in transfected colonies was determined by western blot. The two ER expressing cell lines are designated as MDAER+/C7 and MDA ER+/H6. (D) MDAER+/C7 cells secrete 9-fold less IL-6 and MDAER+/H6 cells secrete 12-fold less IL-6 in response to OSM compared to the parental MDA-MB-231 cells. Data expressed as mean ± SEM and significance assessed by one-way ANOVA with Tukey’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001.