Table 1.
Tilapia primer sequences used for quantitative PCR
| Gene | Forward Primer (5′–3′) | Reverse Primer (5′–3′) | Accession No. |
|---|---|---|---|
| SGLT1 | CCCGAGTACTTGAAGAAGAG | GCAATAACAGCGAGGTAGA | XM_019361130.1 |
| SGLT2 | AGGAGGATGAGGGAGAATAA | ACACTGGGTCCTCACTAA | XM_013273846.2 |
| SMIT1 | CAACGCCAAGTACAGAAAC | CTCTGCGATTCTCCCTTATAG | XM_019353114.1 |
| SGLT4 | ATGGTGGTTGGAATCTGG | GTCCCTGCTAGACCAATAAA | XM_005475685.3 |
| NIS | TCCATGTCCTACCTCTACTT | GACCCTCCACTTTCTTCTT | XM_005453978.3 |
| SMCT1 | TCAGTGTTGAGTGAGAAGG | AGAGCGAACACCACATAG | XM_013271289.2 |
| SGLT5 | CACCGTGGACTCTGATTT | TACATCTGCGTGTGAAGAG | XM_003455975.4 |
| SMIT2 | GAGACGGAAGAAGGAAGATG | GCCCAGTAACCAATGATAAAG | XM_005461087.3 |
| SMIT | GCTGTCTGTGGATCTCTATT | GAACTGTGTCCGTGTAGAT | XM_019352280.1 |
| SMVT | CAGGAGGAATAGCAGAAGTC | CTTGGTTCACACCGTACA | XM_005460242.3 |
| EFα1 | CTCTTCTACCGTCGGATTAC | ATTGACTCCCTCGTAGAAAC | XM_005476483.3 |
EFα1, α-elongation factor 1; NIS, sodium-iodide cotransporter; SGLT1, SGLT2, SGLT4, SGLT5, sodium-glucose cotransporters 1, 2, 4, and 5, respectively; SMCT1, sodium-monocarboxylate cotransporter 1; SMIT, SMIT1, SMIT2, sodium-myoinositol cotransporter and cotransporters 1 and 2, respectively; SMVT, sodium-multivitamin cotransporter.