Table 3.
Nile Tilapia |
Rainbow Trout |
|||||
---|---|---|---|---|---|---|
Tilapia (Vmax)/Trout (Vmax), % | Tissue | Vmax, µA/cm2 | Km, mM | Tissue | Vmax, µA/cm2 | Km, mM |
Proximal | 47.0 ± 6.7 | 4.2 ± 0.7 | Pyloric ceca | 2.3 ± 0.4a,b | 4.2 ± 1.0a | 2,043d |
Midintestine | 59.6 ± 7.0 | 6.0 ± 1.0 | Midgut | 1.7 ± 0.3a | 1.8 ± 0.3b | 3,506d |
Hindgut | 56.2 ± 9.3 | 5.7 ± 1.1 | Hindgut | 2.4 ± 0.2b | 12.3 ± 1.6c | 2,342d |
Values are means ± SE. Maximal velocity (Vmax; µA/cm2) and Michaelis-Menten constant (Km; mM) are represented for each tissue type in Nile tilapia and rainbow trout. Area of intestinal segment was 0.3 cm2. There were no significant differences in Vmax and Km values between tissues for tilapia. In trout, significant differences between Vmax values are as follows:
pyloric ceca vs.
hindgut, and a midgut vs. bhindgut, with no significant differences between pyloric ceca and midgut (Tukey’s test after one-way ANOVA, P < 0.05). Significant differences between Km values in trout are as follow: apyloric ceca vs. bmidgut, apyloric ceca vs.
Significant Vmax values between tilapia and trout (Student’s t-test, P < 0.05).