Fig 3. Knockdown of Src-1 or Twist1 expression in CNE-1 upregulates E-cadherin expression and downregulates Vimentin expression.
(A, B) 2.5×105 CNE-1 cells were seeded in 6-well-plates and transduced with sh-Src-1, sh-Twist1 or a negative control (sh-NC) for 4 days, then cell lysates were analyzed by western blot. (C, D) quantification of western blot bands in A and B. Protein expression was normalized to GAPDH. Lysates of parental CNE-1 cells were used as positive control. ***P<0.05 comparing with sh-NC group.