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. 2019 Apr 11;14(4):e0215208. doi: 10.1371/journal.pone.0215208

Fig 4. SIRT1 requires HDAC1 to protect CGNs against apoptosis.

Fig 4

A. SIRT1 requires HDAC1. GFP or SIRT1-Flag were co-expressed in a 1:2 ratio with either a control shRNA (pLK0.1) or a distinct HDAC1 shRNA (sh A) (**p<0.01 and ***p<0.001 as compared to GFP/pLK0.1 treated with LK; ##p<0.01 as compared to SIRT1/pLK0.1 treated with LK; n = 3). B. SIRT1 and Δ9, but not Δ8, rescue HDAC1 toxicity. CGNs were transfected with GFP or co-transfected with HDAC1-GFP and either a control vector (Ctrl, 3xFLAG), SIRT1-Flag, Δ8-Flag, or Δ9-Flag in a 1:2 ratio (*p<0.05, **p<0.01, ***p<0.001; n = 3).