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. 2019 Apr 11;14(4):e0215208. doi: 10.1371/journal.pone.0215208

Fig 5. SIRT1 does not require HDAC1 activity.

Fig 5

A. CGNs were expressed with GFP alone or co-expressed with SIRT1, Δ5, Δ6, or Δ9 in a 1:2 ratio with GFP or Δ56 and then treated as indicated (***p<0.001; n = 3). B. SIRT1-Flag or HDAC3-Flag were each expressed in a 1:1 ratio with either HDAC1-GFP or Δ56-GFP into HEK293T cells for 48 h after which immunoprecipitation was performed with either and IgG or Flag antibody. Western blots were initially probed for GFP and subsequently probed for Flag. Graph shows densitometry from four independent experiments. C. Δ56 was expressed into HEK293T cells in a 1:1 ratio with SIRT1 or Δ1-Δ10 for 48 h. Immunoprecipitation was performed as described in (B). Graph shows densitometry (one-way ANOVA with Dunnett’s multiple comparisons posttest) from five independent experiments. CGNs were co-transfected with GFP or Δ56-GFP and either a control vector (Ctrl, 3xFLAG), SIRT1-Flag, Δ8-Flag, or Δ9-Flag in a 1:2 ratio (*p<0.05, **p<0.01; n = 3).