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. 2019 Apr 11;14(4):e0215064. doi: 10.1371/journal.pone.0215064

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© 2019 Lien et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) pop2Δ mutant cells harboring YCplac33-POP2FLAG (POP2FLAG), YCplac33-POP2S39AFLAG (POP2S39AFLAG) were grown at 30°C until exponential phase. Extracts prepared from each strain were run on phos-tag and conventional gels, then immunoblotted with anti-Flag antibody. Phosphorylated Pop2Flag is indicated with arrows and numbers according to the positions. Band 3 appears as dominant in the pop2Δ mutant harboring the POP2-FLAG plasmid, but less dominant in the WT strain harboring the POP2-FLAG plasmid (see Fig 2B). (B) Wild-type cells harboring YCplac33-POP2FLAG (POP2FLAG) were grown at 30°C until exponential phase. Extracts prepared from this strain were immunoprecipitated using anti-Flag antibody. Immunoprecipitated Pop2Flag was treated with/withoutλphosphatase. The band patterns of immunoprecipitated Pop2 are compared with the band patterns of Pop2 and Pop2S39AT97A extracted with NaOH as analyzed in (A).