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. 2019 Apr 11;14(4):e0215064. doi: 10.1371/journal.pone.0215064

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© 2019 Lien et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) WT and snf1Δ cells harboring YCplac33-POP2FLAG (POP2FLAG, snf1ΔPOP2FLAG) were collected as in Fig 2A. Extracts prepared from each strain were run on phos-tag and conventional gels, then immunoblotted with anti-Flag antibody. Phosphorylated Pop2Flag is indicated with arrows and numbers according to the positions. Representative data are shown. (B) WT and yak1Δ cells harboring YCplac33-POP2FLAG (POP2FLAG, yak1ΔPOP2FLAG) were collected as in Fig 2A. Samples were analyzed as in (A). Representative data are shown.