Fig 3. PT-IC-seq of E. coli B7A genome reveals low PT modification frequency globally.

(A) The snapshot of genome browser representing partially modification site in bacteria genome. The top track shows counts of 5’ ends in the selected region. Blue segments represent reads mapped to minus strand and red segments represent reads mapped to plus strand. The red triangle marks two partial PT sites in E. coli B7A genome (B) The ratio of the I₂-specific-cleavaged reads to all the reads across the site and the PT ratio falling in the range of proportions. Statistics analysis of the PT modification frequency in the genomes of E. coli B7A. All of the PT modified sites in the genomes of E. coli B7A are partially-PT modified, and about 60% of the PT modified sites with the PT ratio lower than 5%.