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(A) Reaction scheme for the “inverted system” involving coupling water-soluble alkyne 4 and hydrophobic azide 5 to give 6. (B) Kinetics using ligands B (blue triangles) and C (red diamonds) in the unseeded inverted system. Identical reaction conditions to those for the original system were applied. (C) Kinetics when the inverted system is seeded with product (30 mM). Points are the mean of three independent experiments and the error bars are the standard deviation.
