. Author manuscript; available in PMC: 2019 Apr 11.

Published in final edited form as: ACS Chem Biol. 2012 Aug 30;7(11):1873–1883. doi: 10.1021/cb300261e

Table 2.

Effect of JNK2 Mutations on the Sensitivity of JNK towards (−)-zuonin A.

Mutants	K_m^b μM	k_cat^b s^-1	k_cat/K_m μM s^-1	IC₅₀^a μM	% Inhibition at 200 μM
JNK2 WT	1.65±0.1	1±0.15	0.6	2.9±0.17	70%
JNK2 (R127A)	23.6±1.1	1.4±0.3	0.06	14±2.1	80%
JNK2 (Y130A)	1.87±0.3	1±0.04	0.53	2.3±0.16	95%
JNK2 (C163A)	1.33±0.4	1±0.065	0.75	37±2.5	37%
JNK2 (T164A)	0.73±0.18	1.1±0.064	1.5	13.6±1.5	39%
JNK2 (W324A)	2.3±0.15	1.06±0.075	0.46	16.6±0.17	98%
JNK2 (D326A)	2±0.83	0.8±0.08	0.4	2.8±0.32	71%

IC₅₀ Determined as described in Methods section, Data were fitted to equation 4

Initial velocity data at different concentrations of c-Jun were fitted to equation 1