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. Author manuscript; available in PMC: 2020 Apr 12.
Published in final edited form as: Circ Res. 2019 Apr 12;124(8):1253–1265. doi: 10.1161/CIRCRESAHA.118.314567

Figure 4. SMC-specific overexpression of PKCδ accelerates re-endothelialization.

Figure 4.

(A) Schematic design of SMC-specific PKCδ expression vector. Expression of PKCδ cDNA and EGFP cDNA is driven by a chimeric promoter (enSM22α) that contains a smooth muscle-myosin heavy chain (SM-MHC) enhancer and the SM22α promoter. (B) Representative images of uninjured carotid arteries or AdenSM22α-PKCδ-EGFP infected arteries. Sections were immunostained for EGFP. Nuclei were stained with DAPI (blue). The locations of the internal and external elastic lamina defining boundaries of the media are shown as white dashed lines. Scale bar = 50 μm. (C) Representative Evans blue-stained carotid arteries harvested 14 days after angioplasty from sham, injured AdenSM22α-EGFP-treated, or injured AdenSM22α-PKCδ-treated rats. Boundaries of the injured areas are indicated by the dashed lines. Reendothelialization was determined by the percentage of Evans blue negative area over the total injured area using ImageJ software. Scale bar = 3 mm. Results are expressed as mean±SEM. n=3-4, *p<0.05, One-way ANOVA.