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. Author manuscript; available in PMC: 2020 Apr 12.
Published in final edited form as: Circ Res. 2019 Apr 12;124(8):1253–1265. doi: 10.1161/CIRCRESAHA.118.314567

Figure 7. PKCδ regulates Cxcl7 expression through STAT3.

Figure 7.

. (A&B) SMCs were infected with Lenti-non-targeting shRNA (shNT) or Lenti-PKCδ shRNA (shPKCδ) (A), AdNull or AdPKCδ (B) followed by incubation with PBS or TNFα for 12h. Levels of Cxcl7 mRNA were analyzed using qPCR. Results are expressed as mean±SEM. n=6, *p<0.05, One-way ANOVA. (C&D) SMCs were transduced with non-targeting siRNA (siNT), STAT3 siRNA (siSTAT3) (C), or STAT1 siRNA (siSTAT1) followed by incubation with PBS or TNFα for 12h. Levels of Cxcl7 mRNA expression were analyzed using qPCR. Results are expressed as mean±SEM. n=5, *p<0.05, One-way ANOVA. (E) SMCs were transduced with indicated siRNA and adenoviral vectors followed by incubation with PBS or TNFα for 12h, Cxcl7 mRNA expression was analyzed using qPCR. Results are expressed as mean±SEM. n=4, *p<0.05, One-way ANOVA. (F&G) TNFα induced STAT3 S727 phosphorylation in SMCs infected with lentivirus shNT or shPKCδ (F) and in SMCs infected with adenovirus AdNull or AdPKCδ (G). Cells were harvested at the indicated time and whole-cell lysates were subjected to immunoblot analysis. n=3.