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. 2019 Feb 21;411(11):2327–2338. doi: 10.1007/s00216-019-01673-w

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© The Author(s) 2019

OpenAccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 6 — Overloading effect. The peaks are normalized for visual comparison; AF4 conditions: ${\dot{V}}_{c}$ = 3.0 mL/min, ${\dot{V}}_{out}$ = 1.5 mL/min, RC 10 kDa