Table 1.

Physicochemical properties of the standard proteins used in this study

Protein	MW (kDa)	D (m2 s−1) b	RH (nm) c	pI
β-Lactoglobulin	36.7 a	8.04·10−11	2.8	5.1, 5.3 [38]d
BSA	66.5	6.21·10−11 [36]	3.6	4.7 [36]
γ-Globulin	150	4.22·10−11	5.3	6.5 (average) [39]
Apoferritin	443	3.31·10−11	6.8	4.0 [40]
Thyroglobulin	669	2.43·10−11	9.2	4.6 [41]

^aThe MW of the β-lactoglobulin monomer is 18.35 kDa but it is present as a dimer in neutral pH [42]

^bDiffusion coefficients were estimated experimentally using BSA as calibrant

^cHydrodynamic radii were estimated from the Stokes–Einstein equation

^dThe pI of β-lactoglobulin is 5.1 and 5.3 for the variants A and B respectively [38]