Table 3.
Retention time and plate height under low (10 μg) and high (50 μg) injected sample mass, for mean layer thickness ℓ = 3 μm and retention time tR = 4 min. The average RSD for the retention time is 3% and for the plate height is 4%
| tR (min) | H (mm) | Increase in H (mm) ± s.d. | |||
|---|---|---|---|---|---|
| Protein | 10 μg | 50 μg | 10 μg | 50 μg | |
| Regenerated cellulose (RC) 10 kDa | |||||
| β-Lactoglobulin | 4.03 | 4.29 | 0.49 | 0.86 | 0.37 ± 0.03 |
| BSA | 3.80 | 3.89 | 0.46 | 0.55 | 0.08 ± 0.01 |
| γ-Globulin | 3.91 | 4.44 | 0.77 | 1.71 | 0.92 ± 0.02 |
| Apoferritin | 3.86 | 3.92 | 0.76 | 0.89 | 0.12 ± 0.01 |
| Thyroglobulin | 4.02 | 4.14 | 0.97 | 1.20 | 0.21 ± 0.08 |
| Polyethersulfone (PES) 5 kDa | |||||
| β-Lactoglobulin | 4.04 | 4.30 | 0.51 | 0.75 | 0.24 ± 0.03 |
| BSA | 4.08 | 4.23 | 0.48 | 0.63 | 0.16 ± 0.02 |
| γ-Globulin | 4.22 | 5.08 | 0.87 | 1.70 | 0.83 ± 0.02 |
| Apoferritin | 4.11 | 4.33 | 0.91 | 0.99 | 0.08 ± 0.06 |
| Thyroglobulin | 4.21 | 4.59 | 1.10 | 1.30 | 0.21 ± 0.01 |