Fig. 2.

Activation of mTOR and its downstream effectors in the ACC after nerve injury. a–c Expression levels of p-mTOR, p-p70S6K, and p-4E-BP1 after nerve injury. Levels of p-mTOR, p-p70S6K, and p-4E-BP1 gradually increased after nerve injury as shown by western blot analysis. Seven days after nerve injury (POD 7), p-mTOR, p-p70S6K, and p-4E-BP1 expression were significantly up-regulated compared with the normal group, but all phospho-protein levels declined 14 days after nerve injury (POD 14) as compared with the POD 7 group. Total protein levels in all groups did not change over time. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01 vs. normal, one-way ANOVA followed by Tukey’s multiple comparison test