Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 11;9:5922. doi: 10.1038/s41598-019-42362-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Implantation of iPSC-derived pancreatic exocrine cells into the gastric submucosal space of rat. (a) Haematoxylin-eosin staining of the implanted cells. The implanted cells were observed in the submucosal space of the stomach (arrowhead), and the implanted pancreas directly attached to the gastric mucosa without the interference of muscularis mucosa (arrow). Scale bar: 100 μm. (b) Representative images of immunofluorescence staining at the implantation site. Top: human mitochondria (green), amylase (red), and trypsin (white). Bottom: human mitochondria (green) and cytokeratin 19 (red). Nuclei were stained with Hoechst 33258 (blue). The implanted iPSC-derived exocrine pancreas still expressed amylase and trypsin. Scale bar: 100 μm. (c) Amylase level in the gastric juice. Amylase in the gastric juice was elevated in two out of four implanted rats, while there was no elevation in the other 2 rats (n = 4). The elevation of amylase was not detected in the gastric juice of the control group (n = 2).