Table 1.
Uridylated non-guide RNA; 49-nt | AAAAAAAAAAAAAAATAGTGATATCGAATTCCTTAGTATGTATCTGGTACCCTATAGTGACTCCTATTA |
CYb edited RNA | TAAAAAGACAACATAAATTTCTAAATAATAAAAAAAATAACAAAAATCTAACACGAAAAAACATATTTCCCTATAGTGAGTCGTATTA |
Poly AU RNA | AATAAGAGAGAAAAAATAAATAAATAAATAAATAAAAGAGACTCGAAAAGAATCCCTATAGTGAGTCGTATTA |
Poly AU mutant RNA | AATAAGAGAGAAAAAATAGATAGATAGATAGATAAAAGAGACTCGAAAAGAATCCCTATAGTGAGTCGTATTA |
42-mer CYb guide RNA | AAAAGCGGAGAAAAATTCACATTTCTTTTAATGTCAGTCCCCCCTATAGTGAGTCGTATTA |
Natural guide CYb RNA | AAAAAAAAAAAAAAAATTATTCCCTTTATTACCTTAAGAAATTCACATTGTCTTTTAATCCCTATAGTGAGTCGTATTA |
ga6(14) gRNA without U-tail | AATAATTATCATATCACTGTCAAAATCTGATTCGTTATGGAGTTATAGTATATTCCCCCTATAGTGAGTCGTATTA |
Underlined sequences represent T7 promoter sequence.