Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 11;9:5926. doi: 10.1038/s41598-019-42251-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Drug sensitivity in various cell types. Cells were treated as indicated and viability determined by methylene blue staining. Bars represent averages and associated standard deviations. (A) Drug sensitivity in cancer cell lines differing in p53 status. Parental HCT116 with wild-type p53 were compared to p53-null HCT116 cells. Cells were exposed to 10 μM erastin, 10 μM compound 4, or 300 μM sulfasalazine for 3 days before viability was determined. 4 was tested in parallel on NCI-H522 to confirm activity. 4 toxicity was also assessed in parental HT1080 cells with wild-type p53, and cells overexpressing a dominant negative p53 fragment, GSE56. Cells were exposed to 10 μM compound 4 in the presence or absence of βME and viability determined. (B) Effect of Liproxstatin. NCI-H522 and HCT116 cells were exposed to 10 μM of either compound 4 or Erastin in the presence or absence of 2.5 μM liproxstatin. Viability was determined 3 days later. Excess glutamate (5 mM) had no effect on cell viability. (C) NCI-H522 cells depend on external cystine. Cells were grown in medium lacking cystine supplemented with dialyzed fetal bovine serum for 3 days. Additional compounds were added and viability determined. (D) Dose response of external cystine. NCI-H522 cells were grown for 3 days in the indicated concentrations of cystine and viability determined. (E) Effects of 1 and analogues on cell viability. The cell types indicated were exposed to the indicated analogues for 2 days and viability determined. MDAH041 are a spontaneously immortalized p53-null human fibroblast cell line^51,52. NCI-H522 were exposed to classical chemotherapy drugs for 2 days (HU: 2 mM, TX: 10 μM, AD: 0.2 μg/ml). (F) Effects of various compounds on WI38, MDAH041 and retinal pigmented epithelial (RPE) cells. Cells were exposed 4, sulfasalazine (300 μM) or erastin (10 μM) 3 days and viability determined. (SSZ: sulfasalazine; βME: β-mercaptoethanol; LIP: liproxstatin; ERA: erastin; GLU: glutamate; CPO: ciclopirox olamine; TRO: trolox; FER: ferrostatin).