Fig. 2. SC66 inhibits Akt signaling and expression of COL11A1, TWIST1, and Mcl-1 in ovarian cancer cells.

a The protein expression levels of p-Akt, Akt, and COL11A1 in a panel of eight ovarian cancer cell lines were evaluated by western blotting. β-Actin protein was used as an internal loading control. All experiments were performed in triplicate. b The half maximal inhibitory concentration (IC₅₀) value (mean ± SD) of SC66 in ovarian cancer cells was measured using the MTT assay. c, d The protein expression levels of p-Akt, Akt, p-p70S6K, p70S6K, p-4EBP1, 4EBP1, COL11A1, TWIST1, and Mcl-1 in cells treated with different concentrations of SC66 for 24 h were evaluated by western blotting. β-Actin was used as an internal loading control. All experiments were performed in triplicate